Keywords
Abstract
The range of normal or expected hematological and clinical biochemical parameters in laboratory animals can be a useful tool for interpreting toxicological studies. However, such information is often difficult to find. Also, the normal values of hematological and clinical biochemical parameters can vary in different lines of laboratory animals and among animals of different nurseries, and also to some extent depend on the research methods used. The purpose of this study was a retrospective assessment of the clinical biochemistry data of healthy rats (control groups) from the nursery of the SI «Institute of Pharmacology and Toxicology of the National Academy of Medical Sciences of Ukraine». Results (from 11 studies that have been conducted in recent years in SI «Institute of Pharmacology and Toxicology of the National Academy of Medical Sciences of Ukraine») on male Wistar rats serum blood investigations were collected and analyzed for this purpose. The data obtained on male rats were used for analysis because most studies were conducted on animals of this sex. Before determining any pathological changes in the organism of experimental animals, reliable control range values should be obtained. Such data can be obtained from control groups during each study. It is believed that various analytical methods, as well as variables related to the environment and technology, influence the values obtained for each particular parameter. Since the data presented in this publication was obtained by the only instrument of testing, and the animals come from the same source, certain risks were minimized. The collection and analysis of clinical biochemical parameters of male Wistar rats’ blood serum (control groups from 11 studies) showed that all parameters are within the normal range of clinically healthy rats. The historical data obtained can be used as control results (norms) to optimize studies in institutions that conduct experiments using rats, in particular, preclinical assessment of the efficacy and safety of drugs and other toxicological studies. The results included in this publication are for informational purposes only and not for the final interpretation of any toxicological research results. They can be useful in explaining why specific statistically significant results of the study have no biological or toxicological significance, for assessing the background pathology in laboratory rat populations, or for identifying analytical problems in the laboratory.