Keywords
Abstract
One of the problems in the treatment of comorbid conditions is associated with the identification of factors that could predict the effectiveness of the treatment of osteoarthritis and hypothyroidism. It is known that one of these factors is the determination of the level of matrix metalloproteinases. It is known that hypofunction of the thyroid gland leads to metabolic disorders that negatively affect the condition of bone and cartilage, causing the development of osteoarthritis. Although NSAIDs are effective in reducing pain and inflammation in patients with osteoarthritis, but it is still unclear to what extent these drugs can affect joint metabolism and, therefore, joint structure, especially against the background of functional thyroid insufficiency. The aim of the study – to research the changes in the level of matrix metalloproteinases 3, 9 and 13 under the influence of NSAIDs and paracetamol at experimental equivalents of osteoarthritis and hypothyroidism. The experiments were performed on 75 white non-linear rats of both sexes with model of osteoarthritis and hypothyroidism. Experimental osteoarthritis was performed by single intra-articular administration of 0.1 ml of monoacetic acid solution in the knee joint (3 mg of the reagent on 50 μl of sterile physiological saline). Experimental hypothyroidism was modeled by enteral administration of a 0,02 % solution of carbimazole, for 6 weeks. The adequacy of the model was confirmed by the level of serum TSH, T3 and T4 in rats. After the formation of the experimental models on the 42 day of the experiment, the animals were divided into 14 groups and started drug administration (NSAIDs and paracetamol) for 5 days. The quantitative level of matrix metalloproteinases in blood serum was determined by competitive in vitro ELISA twice – on 42 and 47 days of the experiment. Blood samples were obtained from the rat tail vein by puncture using a vacuum system at 42 and 47 days of the experiment. Statistical data processing was performed. An analysis of the results found that changes in the level of matrix metalloproteinases in rat blood serum under the influence of different NSAIDs and paracetamol are not the same. Based on the study, it was found that the determination of the level of matrix metalloproteinases allows one to evaluate the antiinflammatory activity of NSAIDs under experimental equivalents of osteoarthritis and hypothyroidism. According to the degree of influence on degenerative-dystrophic processes in bone and cartilage tissue, the studied drugs can be positioned as follows: diclofenac sodium > ibuprofen > nimesulide = meloxicam > celecoxib > paracetamol.